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mim_ramm_vts [2023/07/31 13:10] – [MIM Tube Lenses and Assemblies] jonmim_ramm_vts [2026/04/28 19:29] (current) – [MIM Tube Lenses and Assemblies] changed CA of TUBE-B and TUBE-180L without C60-RING jon
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 With infinity microscope systems, the spacing between the objective and tube lens doesn't change the optical magnification.  This “infinity space” or "collimated space" provides a region where other optical systems can be coupled to the microscope relatively easily.  For epi-fluorescent illumination, a filter cube with a dichroic beam splitter can be added to provide the illumination path.  If the infinity space is too long then vignetting can occur; see the section [[mim_ramm_vts#infinity_space_limitations|Infinity Space Limitations]] (the allowable length scales with the tube lens focal length, so this is most troublesome when trying to decrease magnification by using a short focal length tube lens). With infinity microscope systems, the spacing between the objective and tube lens doesn't change the optical magnification.  This “infinity space” or "collimated space" provides a region where other optical systems can be coupled to the microscope relatively easily.  For epi-fluorescent illumination, a filter cube with a dichroic beam splitter can be added to provide the illumination path.  If the infinity space is too long then vignetting can occur; see the section [[mim_ramm_vts#infinity_space_limitations|Infinity Space Limitations]] (the allowable length scales with the tube lens focal length, so this is most troublesome when trying to decrease magnification by using a short focal length tube lens).
  
-For imaging applications the most common tube lenses are the ''C60-TUBE-B'' which uses a Nikon 200mm multi-element tube lens, the ''Cxx-TUBE-200NTi'' which uses the same tube lens as the Nikon Ti-2 microscope, or the ''C60-TUBE-180L'' which uses the Olympus 180mm multi-element tube lens.  The Ti-2 tube lens has the largest clear aperture and large field but is also significantly more expensive than the others.  The Olympus ''C60-TUBE-180L'' is designed for a larger field than the ''C60-TUBE-B'' but is slightly more expensive.+For imaging applications the most common tube lenses are the ''C60-TUBE-B'' which uses a Nikon 200mm multi-element tube lens, the ''Cxx-TUBE-200NTi'' which uses the same tube lens as the Nikon Ti-2 microscope, or the ''C60-TUBE-180L'' which uses the Olympus 180mm multi-element tube lens.  The ''Cxx-TUBE-200NTi'' tube lens has the largest clear aperture and large field but it is also significantly more expensive than the others.  The Olympus ''C60-TUBE-180L'' is designed for a larger field than the ''C60-TUBE-B'' but is slightly more expensive.
  
 ASI historically used VIS coatings for most of the achromat tube lenses (425-675nm), but since ~2015 we have gradually transitioned to using VIS-NIR coatings (400-100nm) to have versatility at the expense of slightly lower transmission.  This transition is mostly complete as of 2022, and please contact us if you have special requirements.  The OEM tube lenses and a few of the others have special coatings as indicated in the table below. ASI historically used VIS coatings for most of the achromat tube lenses (425-675nm), but since ~2015 we have gradually transitioned to using VIS-NIR coatings (400-100nm) to have versatility at the expense of slightly lower transmission.  This transition is mostly complete as of 2022, and please contact us if you have special requirements.  The OEM tube lenses and a few of the others have special coatings as indicated in the table below.
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 All tube lenses have a 50 mm O.D. Most tube lenses have a 30 mm I.D. on the focus side with a 60 mm distance from the end of the tube to the image plane. Tubes ending with "-M" are different. "-M" lenses are female C60 on the focus side with varying distances to the image plane. The table below describes the various tube lenses with dimensions as indicated in Figure 1. All tube lenses have a 50 mm O.D. Most tube lenses have a 30 mm I.D. on the focus side with a 60 mm distance from the end of the tube to the image plane. Tubes ending with "-M" are different. "-M" lenses are female C60 on the focus side with varying distances to the image plane. The table below describes the various tube lenses with dimensions as indicated in Figure 1.
  
-^ Part Number    ^ Lens F.L. (mm)  ^ Relative Magnification                          |^  Lens type                                                        ^ BFL (mm) ((Note that this is distance from flange to the focal plane in **collimated space**, which is an unconventional definition of BFL )) ^ LOA (mm)  ^  CA (mm) ((Clear aperture of C60-RING is 30 mm which may limit, use C60-RING-A-34 for 34 mm clear aperture if necessary))  ^   +^ Part Number    ^ Lens F.L. (mm)  ^ Relative Magnification                          |^  Lens type                                                        ^ BFL (mm) ((Note that this is distance from flange to the focal plane in **collimated space**, which is an unconventional definition of BFL )) ^ LOA (mm)  ^  CA (mm) ((Clear aperture of C60-RING is 30 mm which may limit, use C60-RING-A-34 for 34 mm clear aperture if necessary)) ^ Collimated-side connection ^ Focus-side connection ^ Flange to Focal Plane (mm) ^ 
-| :::            | :::             ^ Nikon Objectives  ^ Olympus Objectives  | :::                                                               | :::       | :::      |  ::: | +| :::            | :::             ^ Nikon Objectives  ^ Olympus Objectives  | :::                                                               | :::       | :::      |  ::: | :::      |  ::: | ::: | 
-^ C60-TUBE-B     | 200             | 1.00              | 1.11                | Nikon OEM multi-element tube lens, coating ~VIS-NIR                                     | 230       | 126.2   |  30  | +^ C60-TUBE-B     | 200             | 1.00              | 1.11                | Nikon OEM multi-element tube lens, coating ~VIS-NIR                                     | 230       | 126.2   |  33  | C60 female | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-B-M     | 200             | 1.00              | 1.11                | Nikon OEM multi-element tube lens, coating ~VIS-NIR, 138.5mm to focal plane                                     | 230       | 47.7   |  30  | +^ C60-TUBE-B-M     | 200             | 1.00              | 1.11                | Nikon OEM multi-element tube lens, coating ~VIS-NIR | 230       | 47.7   |  33  | C60 female | C60 female | 138.5 | 
-^ C60-TUBE-200NTi     | 200             | 1.00              | 1.11                | Nikon Ti-2 tube lens, note **C60** version with C60-RING-A-34                                     | 183       | 162.3   |  34  | +^ C60-TUBE-200NTi     | 200             | 1.00              | 1.11                | Nikon Ti-2 tube lens, **C60** version includes C65M-C60F-ADPT and C60-RING-A-34                                     | 183       | 162.3   |  34  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C65-TUBE-200NTi     | 200             | 1.00              | 1.11                | Nikon Ti-2 tube lens, note **C65** version                                     | 189       | 156.3   |  36  | +^ C65-TUBE-200NTi     | 200             | 1.00              | 1.11                | Nikon Ti-2 tube lens, note **C65** version                                     | 189       | 156.3   |  36  | //C65// female | 30mm/Zeiss | 60.0 | 
-^ C65-TUBE-200NTi-M     | 200             | 1.00              | 1.11                | Nikon Ti-2 tube lens, note **C65** version, 169mm to focal plane                                     | 189       | 47.3   |  36  | +^ C60-TUBE-200NTi-M     | 200             | 1.00              | 1.11                | Nikon Ti-2 tube lens, note **C60** version with qty 2 C65M-C60F-ADPT and C60-RING-A-34                                     | 183       | 59.3   |  36  | C60 male | C60 female | 163.0 | 
-^ C60-TUBE-180L     | 180             | 0.90              | 1.00                | Olympus OEM multi-element tube lens, coating ~VIS                                     | 181       | 130   |  30  | +^ C65-TUBE-200NTi-M     | 200             | 1.00              | 1.11                | Nikon Ti-2 tube lens, note **C65** version both sides                                     | 189       | 47.3   |  36  | //C65// female | //C65// female | 169.0 | 
-^ C60-TUBE-180L-M     | 180             | 0.90              | 1.00                | Olympus OEM multi-element tube lens, coating ~VIS, 123.9mm to focal plane                                    | 181       | 66   |  30  | +^ C60-TUBE-180L     | 180             | 0.90              | 1.00                | Olympus OEM multi-element tube lens, coating ~VIS                                     | 181       | 130   |  34  | C60 female | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-Z13   | 164.5           | NA                | NA                  | Zeiss OEM multi-element tube lens ((nominal 130mm +/- 5mm distance from objective flange to lens surface, so 121mm +/-5mm from C60-TUBE-Z13 to objective flange.  This is for optimal chromatic correction.))  | 150.5     | 121.1    |  34  | +^ C60-TUBE-180L-M     | 180             | 0.90              | 1.00                | Olympus OEM multi-element tube lens, coating ~VIS                                    | 181       | 66   |  34  | C60 female | C60 //male// | 123.9 | 
-^ C60-TUBE-200LCA   | 200           | 1.00                | 1.11                  | Leica OEM multi-element tube lens ((roughly 50-150mm to objective flange, should double-check this.))  | ??     | 48.6    |  30?  | +^ C60-TUBE-Z13   | 164.5           | NA                | NA                  | Zeiss OEM multi-element tube lens ((like Axio Imager; nominal 130mm +/- 5mm distance from objective flange to lens surface, so 121mm +/-5mm from C60-TUBE-Z13 to objective flange.  This is for optimal chromatic correction.))  | 150.5     | 121.1    |  34  | C60 female | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-70D   | 70              | 0.35              | 0.39                | Dual Achromat, VIS coating                                                     | 68     | 16       |  16.5  | +^ C60-TUBE-200LCA   | 200           | 1.00                | 1.11                  | Leica OEM multi-element tube lens((roughly 50-150mm to objective flange, should double-check this.)); must be used with LCA-C-MOUNT-xxx adapter   | ??     | 121.1    |  30?  | C60 female | Leica-style female | NA | 
-^ C60-TUBE-80   | 80             | 0.40              | 0.44                | Achromat, VIS coating   (400-700nm)                                                        | 84        | 20     |  28  | +^ C60-TUBE-200LCA-M   | 200           | 1.00                | 1.11                  | Leica OEM multi-element tube lens((roughly 50-150mm to objective flange, should double-check this.)); must be used with LCA-C-MOUNT-xxx adapter; normally requires C60-LCA-C-MOUNT   | ??     | 36.9    |  30?  | C60 female | C60 //male// | NA | 
-^ C60-TUBE-100   | 100             | 0.50              | 0.56                | Achromat, VIS-NIR coating                                                          | 92        | 48.7     |  33  | +^ C60-TUBE-70D   | 70              | 0.35              | 0.39                | Dual Achromat, VIS coating                                                     | 68     | 16       |  16.5  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-100FS   | 100             | 0.50              | 0.56                | PCX fused silica lens (for UV illumination)                                                         | ??        | ??     |  33?  | +^ C60-TUBE-80   | 80             | 0.40              | 0.44                | Achromat, VIS or VIS-NIR coating | 84        | 20     |  28  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-100D  | 100             | 0.50              | 0.56                | Dual Achromat, VIS-NIR coating                                                     | 83.5      | 63.9     |  33  | +^ C60-TUBE-100   | 100             | 0.50              | 0.56                | Achromat, VIS-NIR coating                                                          | 92        | 48.7     |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-125D  | 125             | 0.625             | 0.694               | Dual Achromat, VIS-NIR coating                                                    | 104       | 94.1     |  33  | +^ C60-TUBE-100-M | 100             | 0.50              | 0.56                | Achromat, VIS or VIS-NIR coating, 84.6mm to focal plane                                                         | 92       | 25.9    |  33  | C60 male | C60 female | 84.6 | 
-^ C60-TUBE-133C  | 133.3            | 0.667             | 0.741               | Custom dual doublet, VIS-NIR coating | 118 | 98.3   |  33  | +^ C60-TUBE-100FS   | 100             | 0.50              | 0.56                | PCX fused silica lens (for UV illumination)                                                         | ??        | ??     |  33?  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-140D  | 140             | 0.70              | 0.78                | Dual Achromat, VIS-NIR                                                     | 123.5     | 105.3    |  33  | +^ C60-TUBE-100D  | 100             | 0.50              | 0.56                | Dual Achromat, VIS-NIR coating                                                     | 83.5      | 63.9     |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-160   | 160             | 0.80              | 0.89                | Achromat, VIS or VIS-NIR coating                                                         | 152       | 112.3   |  33  | +^ C60-TUBE-120   | 120             | 0.60              | 0.67                | Achromat, VIS-NIR coating                                                          | 116        | 69.5     |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-160-M | 160             | 0.80              | 0.89                | Achromat, VIS or VIS-NIR coating, 147.8mm to focal plane                                                         | 152       | 25.9    |  33  | +^ C60-TUBE-125D  | 125             | 0.625             | 0.694               | Dual Achromat, VIS-NIR coating                                                    | 104       | 94.1     |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-180D  | 180             | 0.90              | 1.00                | Dual Achromat, VIS coating                                                    | 149.6     | 157.0    |  33  | +^ C60-TUBE-133C  | 133.3            | 0.667             | 0.741               | Custom dual doublet, VIS-NIR coating | 118 | 98.3   |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-200   | 200             | 1.00              | 1.11                | Achromat, VIS-NIR coating (UV available)                                                          | 191.3     | 152.7    |  33  | +^ C60-TUBE-133C-M  | 133.3            | 0.667             | 0.741               | Custom dual doublet, VIS-NIR coating | 118 | 49.8   |  33  | C60 male | C60 female | 108.5 | 
-^ C60-TUBE-250   | 250             | 1.25              | 1.39                | Achromat, VIS-NIR coating                                                          | ~237      | 203.6    |  33  | +^ C60-TUBE-140D  | 140             | 0.70              | 0.78                | Dual Achromat, VIS-NIR                                                     | 123.5     | 105.3    |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-265D((various versions available including folded and with AFS))  | 265             | 1.33              | 1.47                | Dual Achromat, VIS-NIR coating                                                     | ~248      | 229    |  33  | +^ C60-TUBE-160   | 160             | 0.80              | 0.89                | Achromat, VIS or VIS-NIR coating                                                         | 152       | 112.3   |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-265D-M  | 265             | 1.33              | 1.47                | Dual Achromat, VIS-NIR coating, 231.5mm to focal plane                                                     | ~248      | 57.5    |  33  | +^ C60-TUBE-160-M | 160             | 0.80              | 0.89                | Achromat, VIS or VIS-NIR coating, 147.8mm to focal plane                                                         | 152       | 25.9    |  33  | C60 male | C60 female | 147.8 | 
-^ C60-TUBE-300   | 300             | 1.50              | 1.67                | Achromat, VIS-NIR coating                                                          | ~291      | 252.0    |  33  | +^ C60-TUBE-170   | 170             | 0.85              | 0.94                | Achromat, VIS or VIS-NIR coating                                                         | 163       | 120.4   |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-358   | 358             | 1.79              | 1.99                | Achromat, VIS-NIR coating                                                          | ~355?      | ~308.0    |  33  | +^ C60-TUBE-180D  | 180             | 0.90              | 1.00                | Dual Achromat, VIS coating; recommend C60-TUBE-180L instead                                                    | 149.6     | 157.0    |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-400   | 400             | 2.00              | 2.22                | Achromat, VIS-NIR coating                                                          | ~391      | 352.0    |  33  | +^ C60-TUBE-200   | 200             | 1.00              | 1.11                | Achromat, VIS-NIR coating (UV available)                                                          | 191.3     | 152.7    |  33  | C60 male | 30mm/Zeiss | 60.0 | 
-^ C60-TUBE-500-M   | 500             | 2.50              | 2.78                | Achromat, VIS-NIR coating, 485.5mm to focal plane                                                          | ~491      | 25.9    |  33  |+^ C60-TUBE-240D  | 240             | 1.20              | 1.33                | Dual Achromat, VIS-NIR coating                                                     | ~225      | 202.1    |  33  | C60 male | 30mm/Zeiss | 60.0 | 
 +^ C60-TUBE-240D-M  | 240             | 1.20              | 1.33                | Dual Achromat, VIS-NIR coating, 218.5mm to focal plane                                                     | ~225      | 43.7    |  33  | C60 male | C60 female | 232.2 | 
 +^ C60-TUBE-250   | 250             | 1.25              | 1.39                | Achromat, VIS-NIR coating                                                          | ~237      | 203.6    |  33  | C60 male | 30mm/Zeiss | 60.0 | 
 +^ C60-TUBE-265D((various versions available including folded and with AFS))  | 265             | 1.33              | 1.47                | Dual Achromat, VIS-NIR coating                                                     | ~248      | 229    |  33  | C60 male | 30mm/Zeiss | 60.0 | 
 +^ C60-TUBE-265D-M  | 265             | 1.33              | 1.47                | Dual Achromat, VIS-NIR coating, 231.5mm to focal plane; note **male** C60 interface                                                     | ~248      | 57.5    |  33  | C60 male | C60 //male// | 232.2 | 
 +^ C60-TUBE-300   | 300             | 1.50              | 1.67                | Achromat, VIS-NIR coating                                                          | ~291      | 252.0    |  33  | C60 male | 30mm/Zeiss | 60.0 | 
 +^ C60-TUBE-358   | 358             | 1.79              | 1.99                | Achromat, VIS-NIR coating                                                          | ~355?      | ~308.0    |  33  | C60 male | 30mm/Zeiss | 60.0 | 
 +^ C60-TUBE-358-M   | 358             | 1.79              | 1.99                | Achromat, VIS-NIR coating                                                          | ~355?      | 25.9    |  33  | C60 male | C60 female | 340.1 | 
 +^ C60-TUBE-400   | 400             | 2.00              | 2.22                | Achromat, VIS-NIR coating                                                          | ~391      | 352.0    |  33  | C60 male | 30mm/Zeiss | 60.0 | 
 +^ C60-TUBE-500-M   | 500             | 2.50              | 2.78                | Achromat, VIS-NIR coating, 485.5mm to focal plane                                                          | ~491      | 25.9    |  33  | C60 male | C60 female | 485.5 |
  
 [{{mim_ramm_6_.jpg?300|C60-Tube-100}}][{{mim_ramm_7_.jpg?300|C60-Tube-200}}] [{{mim_ramm_6_.jpg?300|C60-Tube-100}}][{{mim_ramm_7_.jpg?300|C60-Tube-200}}]
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 ==== Tube Lens Performance ==== ==== Tube Lens Performance ====
  
-Spherical aberrations are usually the main determinant of tube lens performance.  This quantifies the degree to which all parallel rays are focused to the same point axially.  Chromatic aberrations are also important for applications where more than one wavelength is used (e.g. fluorescent samples with both GFP and mCherry labels).+Spherical aberrations are usually the main determinant of tube lens performance.  This quantifies the degree to which all parallel rays are focused to the same point axially.  Chromatic aberrations are also important for applications where more than one wavelength is used (e.g. fluorescent samples with both GFP and mCherry labels).  Finally field curvature can be pronounced especially in the single-achromat tube lenses and especially at short focal lengths.
  
 All ASI's tube lenses are intended for use with infinity-corrected objectives.  Nikon and Olympus objective lenses (and tube lenses) include all corrections internally and can be safely mixed and matched for convenience, to adjust the magnification, etc.  Leica and Zeiss objectives and tube lenses split the chromatic correction between the objective and tube lens in a proprietary way and cannot be mixed without some performance degradation, particularly chromatically. All ASI's tube lenses are intended for use with infinity-corrected objectives.  Nikon and Olympus objective lenses (and tube lenses) include all corrections internally and can be safely mixed and matched for convenience, to adjust the magnification, etc.  Leica and Zeiss objectives and tube lenses split the chromatic correction between the objective and tube lens in a proprietary way and cannot be mixed without some performance degradation, particularly chromatically.
  
-For best imaging performance we recommend either the ''C60-Tube-B'' which uses a Nikon 200 mm focal length multi-element tube lens or the Olympus equivalent ''C60-Tube-180L''.  In general longer focal length lenses will have better performance relative to the diffraction limit, and smaller objective pupils are more forgiving.  Multi-element lenses ''C60-TUBE-265'' and ''C60-TUBE-180D'' are usually satisfactory for imaging depending on objective pupil size and requirements, and sometimes also (in order of decreasing performance relative to the diffraction limit) ''C60-TUBE-140D'', ''C60-TUBE-125D'', and ''C60-TUBE-100D''.  Likewise ''C60-TUBE-250'', ''C60-TUBE-300'', ''C60-TUBE-358'', and ''C60-TUBE-400'' are usually satisfactory for imaging even though they only contain a single doublet simply because of the longer focal length.  ''C60-TUBE-100'' and ''C60-TUBE-70D'' are not recommended for imaging applications but are intended for illumination, and ''C60-TUBE-160'' is marginal for imaging applications.+For best imaging performance we recommend the ''C60-Tube-B'' which uses a Nikon 200 mm focal length multi-element tube lens, the 180mm ''C60-Tube-180L'' with Olympus glass, or else the large-aperture Nikon 200mm ''Cxx-TUBE-200NTi'' which has the largest clear aperture and excellent performance but at a significant price premium. 
 + 
 +In general longer focal length lenses will have better performance relative to the diffraction limit, and smaller objective pupils (back aperture size) are more forgiving. 
 + 
 +The dual-achromat lenses ''C60-TUBE-265D'' and ''C60-TUBE-240D'' are usually satisfactory for imaging depending on objective pupil size and requirements.  Sometimes also (in order of decreasing performance relative to the diffraction limit) the dual achromat lenses ''C60-TUBE-140D'', ''C60-TUBE-125D'', and ''C60-TUBE-100D''.  ''C60-TUBE-133C'' internally uses a pair of custom-designed doublets and particularly with respect to field curvature is notably better than the dual achromats in similar focal length. 
 + 
 +Lenses with long focal length such as ''C60-TUBE-400'', ''C60-TUBE-358'',  ''C60-TUBE-300'', and (sometimes) ''C60-TUBE-250'' are usually satisfactory for imaging even though they only contain a single doublet simply because of the longer focal length 
 + 
 + ''C60-TUBE-100'' and ''C60-TUBE-70D'' are not recommended for imaging applications but are intended for illumination.  ''C60-TUBE-160'' is marginal for imaging applications depending on the size of objective back aperture and sensor size. 
 + 
 +If there is a question about imaging performance of a specific tube lens, please contact ASI with information about the objective being used, sensor size, and wavelength range: we can run optical simulations to predict how the combination will perform relative to the diffraction limit.
  
  
Address: 29391 W Enid Rd. Eugene, OR 97402, USA | Phone: +1 (541) 461-8181
mim_ramm_vts.1690823418.txt.gz · Last modified: by jon