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multi-immersion_objectives [2025/09/29 20:38] – [Spherical and Chromatic Aberrations] jonmulti-immersion_objectives [2026/10/07 14:18] (current) – [Known Safe Media] jon
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 All of these objective lenses have been designed with large working distance.  A unique feature is that the working distance is exactly the same for all media. All of these objective lenses have been designed with large working distance.  A unique feature is that the working distance is exactly the same for all media.
  
-For most objective lenses, the WD depends on the medium's RI due to refraction at the objective/media surface.  Realizing this was going to be a practical issue we specifically designed both objectives to have uniform WD for all RI.  The optical implementation of this feature is straightforward: the first element of the objective is concave with radius of curvature centered on the focal plane.  Thus to first order there is no refraction at that surface and hence the WD is constant for all RI.  For the same reason, there is no intrinsic spherical aberration as the medium RI changes, unlike objective lenses with a flat first surface.+For most objective lenses, the WD depends on the medium's RI due to refraction at the objective/media surface.  Realizing this was going to be a practical issue we specifically designed all of these objectives to have uniform WD for all RI.  The optical implementation of this feature is straightforward: the first element of the objective is concave with radius of curvature centered on the focal plane.  Thus to first order there is no refraction at that surface and hence the WD is constant for all RI.  For the same reason, there is no intrinsic spherical aberration as the medium RI changes, unlike objective lenses with a flat first surface.
  
  
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 Per optical simulations for the 54-10-12 objective lens, the location of the back focal plane referenced to flange is approximately 31.07 mm - 38.80 mm * RI + 9.42 mm * RI^2 (negative inside flange).  Specifically, this is ~4  mm inside the flange for water, ~5.4 mm inside the flange for FocusClear, and ~6.5 mm inside the flange for ethyl cinnamate (RI 1.56). Per optical simulations for the 54-10-12 objective lens, the location of the back focal plane referenced to flange is approximately 31.07 mm - 38.80 mm * RI + 9.42 mm * RI^2 (negative inside flange).  Specifically, this is ~4  mm inside the flange for water, ~5.4 mm inside the flange for FocusClear, and ~6.5 mm inside the flange for ethyl cinnamate (RI 1.56).
  
-Per optical simulations for the 54-12-8 objective lens, the location of the back focal plane referenced to flange is approximately -11.75 mm - 22.90 mm * RI + 5.87 mm * RI^2 (negative inside flange).  Specifically, this is ~31.8  mm inside the flange for water, ~32.5 mm inside the flange for FocusClear, and ~33.2 mm inside the flange for ethyl cinnamate (RI 1.56). The difference in back focal plane position between the two objectives depends on RI, but is to a good approximation the location is 27 mm deeper inside the objective for the 54-12-8 as measured from the flange, or 5 mm closer to the sample.+Per optical simulations for the 54-12-8 objective lens, the location of the back focal plane referenced to flange is approximately -11.75 mm - 22.90 mm * RI + 5.87 mm * RI^2 (negative inside flange).  Specifically, this is ~31.8  mm inside the flange for water, ~32.5 mm inside the flange for FocusClear, and ~33.2 mm inside the flange for ethyl cinnamate (RI 1.56). The difference in back focal plane position between the 54-10-12 and 54-12-8 depends on RI, but is to a good approximation the location is 5 mm closer to the sample for the 54-12-8.
  
 Per optical simulations for the 57-12-5 objective lens, the location of the back focal plane referenced to flange is ~44.0 mm inside the flange for water and ~44.9 mm inside the flange for the design medium which is RC1.52. Per optical simulations for the 57-12-5 objective lens, the location of the back focal plane referenced to flange is ~44.0 mm inside the flange for water and ~44.9 mm inside the flange for the design medium which is RC1.52.
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   * water with salt, sugars, and/or other non-aggressive solutes including routine-use biological buffers   * water with salt, sugars, and/or other non-aggressive solutes including routine-use biological buffers
-  * FocusClear (CLARITY)+  * FocusClear (CLARITY protocol)
   * glycerol   * glycerol
   * CUBIC-1, CUBIC-2, CUBIC-R1, CUBIC-R2   * CUBIC-1, CUBIC-2, CUBIC-R1, CUBIC-R2
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   * TDE (2,2-thiodiethanol)   * TDE (2,2-thiodiethanol)
   * ECi (ethyl cinnamate)   * ECi (ethyl cinnamate)
-  * benzyl benzoate and BB-PEG (PEGASOS)  +  * PEGASOS (benzyl benzoate and BB-PEG) 
-  * BABB+  * BABB (benzyl alcohol and benzyl benzoate)
   * DBE (dibenzyl ether)   * DBE (dibenzyl ether)
   * 100% ethanol   * 100% ethanol
 +  * DMSO (dimethyl sulfoxide)
 +  * formamide
   * other proprietary organic media   * other proprietary organic media
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multi-immersion_objectives.1759192714.txt.gz · Last modified: by jon